The gto_amino_acid_from_fasta converts DNA sequences in FASTA or Multi-FASTA file format to an amino acid sequence.
For help type:
./gto_amino_acid_from_fasta -h
In the following subsections, we explain the input and output paramters.
The gto_amino_acid_from_fasta program needs two streams for the computation, namely the input and output standard. The input stream is a FASTA or Multi-FASTA file.
The attribution is given according to:
Usage: ../../bin/gto_amino_acid_from_fasta [options] [[--] args]
or: ../../bin/gto_amino_acid_from_fasta [options]
It converts FASTA or Multi-FASTA file format to an amino acid sequence (translation).
-h, --help Show this help message and exit
Basic options
< input.mfasta Input FASTA or Multi-FASTA file format (stdin)
> output.prot Output amino acid sequence file (stdout)
Optional
-f, --frame= Translation codon frame (1, 2 or 3)
Example: ../../bin/gto_amino_acid_from_fasta < input.mfasta > output.prot
An example of such an input file is:
>AB000264 |acc=AB000264|descr=Homo sapiens mRNA
ACAAGACGGCCTCCTGCTGCTGCTGCTCTCCGGGGCCACGGCCCTGGAGGGTCCACCGCTGCCCTGCTGCCATTGTCCC
CGGCCCCACCTAAGGAAAAGCAGCCTCCTGACTTTCCTCGCTTGGGCCGAGACAGCGAGCATATGCAGGAAGCGGCAGG
AAGTGGTTTGAGTGGACCTCCGGGCCCCTCATAGGAGAGGAAGCTCGGGAGGTGGCCAGGCGGCAGGAAGCAGGCCAGT
GCCGCGAATCCGCGCGCCGGGACAGAATCTCCTGCAAAGCCCTGCAGGAACTTCTTCTGGAAGACCTTCTCCACCCCCC
CAGCTAAAACCTCACCCATGAATGCTCACGCAAGTTTAATTACAGACCTGAA
>AB000263 |acc=AB000263|descr=Homo sapiens mRNA
ACAAGATGCCATTGTCCCCCGGCCTCCTGCTGCTGCTGCTCTCCGGGGCCACGGCCACCGCTGCCCTGCCCCTGGAGGG
TGGCCCCACCGGCCGAGACAGCGAGCATATGCAGGAAGCGGCAGGAATAAGGAAAAGCAGCCTCCTGACTTTCCTCGCT
TGGTGGTTTGAGTGGACCTCCCAGGCCAGTGCCGGGCCCCTCATAGGAGAGGAAGCTCGGGAGGTGGCCAGGCGGCAGG
AAGGCGCACCCCCCCAGCAATCCGCGCGCCGGGACAGAATGCCCTGCAGGAACTTCTTCTGGAAGACCTTCTCCTCCTG
CAAATAAAACCTCACCCATGAATGCTCACGCAAGTTTAATTACAGACCTGAA
The output of the gto_amino_acid_from_fasta program is an amino acid sequence.
Using the input above, an output example for this is the following:
TRRPPAAAALRGHGPGGSTAALLPLSPAPPKEKQPPDFPRLGRDSEHMQEAAGSGLSGPPGPS-ERKLGRWPGGRKQAS
AANPRAGTESPAKPCRNFFWKTFSTPPAKTSPMNAHASLITDLTRCHCPPASCCCCSPGPRPPLPCPWRVAPPAETASI
CRKRQE-GKAAS-LSSLGGLSGPPRPVPGPS-ERKLGRWPGGRKAHPPSNPRAGTECPAGTSSGRPSPPANKTSPMNAH
ASLITDL